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datp  (New England Biolabs)


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    Structured Review

    New England Biolabs datp
    Datp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 333 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/datp+solution/dATP+Solution/bio_rxiv__64898__2026__04__30__722071-422-56-57
    Average 97 stars, based on 333 article reviews
    datp - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    other:

    Article Title: The twist-and-squeeze activation of CARF-fused adenosine deaminase by cyclic oligoadenylates
    Article Snippet: dATP solution , New England Biolabs , N0440S.

    In Situ:

    Article Title: DROSHA, DICER, and damage-induced long ncRNA control BMI1-dependent transcriptional repression at DNA double-strand break.
    Article Snippet: Coverslips were then washed twice in 1× rCutSmart buffer (NEB; #B6004S) and twice in 1×T4 ligase buffer (NEB; #B0202S). .. Then, in situ ligation was performed overnight at 16◦C in a sealed humid chamber, in 100 μL final volume per coverslip using: 2 μL T4 Ligase (NEB; #M0202S), 5 μL 10 μM biotinylated linker (5′-TACTACCTCG AGAGTTACGCTAGGGA- TAACAGGGTAATATAGTTT[biotin–dT]TTTCTATATTACCCTGTTA- TCCCTAGCGTAACTCTCGAGGTAG TA-3′), 10 μL 10× T4 Ligase Buffer (NEB; #B0202S), 1 μL dATP solution 100 mM (NEB; #N0440S), 1 μL BSA (20 mg/mL; NEB; 24 Cell Reports 44, 116605, December 23, 2025 #B9200S) and 81 μL H2O. ..

    Article Title: Rewiring of 3D chromatin topology orchestrates transcriptional reprogramming in muscle fiber-type specification and transformation
    Article Snippet: The SDS was quenched by the addition of 145 μl H 2 O and 12.5 μl 20% Triton X-100, and the genome was digested overnight at 37 °C with 100U AluI (NEB, R0137V) into blunt-end fragments. .. Cleaved chromatin was A-tailed by adding 10 mM dATP solution (NEB, N0440S) and Klenow Fragment (3′ → 5′ exo-) (NEB, M0212L) with rotation at 37 °C for 40 min. Then, chromatin was treated with adenine and ligated with a biotinylated bridge linker (F: CGCGATATC/bio-T/TATCTGACT; R: GTCAGATAAGATATCGCGT) at 16 °C for 4 h. After ligation, the nuclei with in situ proximity ligation were treated with l-exonuclease (NEB, M0262S) and exonuclease I (NEB, M0293V) for 1 h at 37 °C to remove noise DNA contain no ligation DNA or bridge linker. ..

    Article Title: DROSHA, DICER, and damage-induced long ncRNA control BMI1-dependent transcriptional repression at DNA double-strand break
    Article Snippet: Coverslips were then washed twice in 1× rCutSmart buffer (NEB; #B6004S) and twice in 1×T4 ligase buffer (NEB; #B0202S). .. Then, in situ ligation was performed overnight at 16°C in a sealed humid chamber, in 100 μL final volume per coverslip using: 2 μL T4 Ligase (NEB; #M0202S), 5 μL 10 μM biotinylated linker (5′-TACTACCTCGAGAGTTACGCTAGGGA- TAACAGGGTAATATAGTTT[biotin–dT]TTTCTATATTACCCTGTTA- TCCCTAGCGTAACTCTCGAGGTAGTA-3′), 10 μL 10× T4 Ligase Buffer (NEB; #B0202S), 1 μL dATP solution 100 mM (NEB; #N0440S), 1 μL BSA (20 mg/mL; NEB; #B9200S) and 81 μL H2O. ..

    Ligation:

    Article Title: DROSHA, DICER, and damage-induced long ncRNA control BMI1-dependent transcriptional repression at DNA double-strand break.
    Article Snippet: Coverslips were then washed twice in 1× rCutSmart buffer (NEB; #B6004S) and twice in 1×T4 ligase buffer (NEB; #B0202S). .. Then, in situ ligation was performed overnight at 16◦C in a sealed humid chamber, in 100 μL final volume per coverslip using: 2 μL T4 Ligase (NEB; #M0202S), 5 μL 10 μM biotinylated linker (5′-TACTACCTCG AGAGTTACGCTAGGGA- TAACAGGGTAATATAGTTT[biotin–dT]TTTCTATATTACCCTGTTA- TCCCTAGCGTAACTCTCGAGGTAG TA-3′), 10 μL 10× T4 Ligase Buffer (NEB; #B0202S), 1 μL dATP solution 100 mM (NEB; #N0440S), 1 μL BSA (20 mg/mL; NEB; 24 Cell Reports 44, 116605, December 23, 2025 #B9200S) and 81 μL H2O. ..

    Article Title: Rewiring of 3D chromatin topology orchestrates transcriptional reprogramming in muscle fiber-type specification and transformation
    Article Snippet: The SDS was quenched by the addition of 145 μl H 2 O and 12.5 μl 20% Triton X-100, and the genome was digested overnight at 37 °C with 100U AluI (NEB, R0137V) into blunt-end fragments. .. Cleaved chromatin was A-tailed by adding 10 mM dATP solution (NEB, N0440S) and Klenow Fragment (3′ → 5′ exo-) (NEB, M0212L) with rotation at 37 °C for 40 min. Then, chromatin was treated with adenine and ligated with a biotinylated bridge linker (F: CGCGATATC/bio-T/TATCTGACT; R: GTCAGATAAGATATCGCGT) at 16 °C for 4 h. After ligation, the nuclei with in situ proximity ligation were treated with l-exonuclease (NEB, M0262S) and exonuclease I (NEB, M0293V) for 1 h at 37 °C to remove noise DNA contain no ligation DNA or bridge linker. ..

    Article Title: DROSHA, DICER, and damage-induced long ncRNA control BMI1-dependent transcriptional repression at DNA double-strand break
    Article Snippet: Coverslips were then washed twice in 1× rCutSmart buffer (NEB; #B6004S) and twice in 1×T4 ligase buffer (NEB; #B0202S). .. Then, in situ ligation was performed overnight at 16°C in a sealed humid chamber, in 100 μL final volume per coverslip using: 2 μL T4 Ligase (NEB; #M0202S), 5 μL 10 μM biotinylated linker (5′-TACTACCTCGAGAGTTACGCTAGGGA- TAACAGGGTAATATAGTTT[biotin–dT]TTTCTATATTACCCTGTTA- TCCCTAGCGTAACTCTCGAGGTAGTA-3′), 10 μL 10× T4 Ligase Buffer (NEB; #B0202S), 1 μL dATP solution 100 mM (NEB; #N0440S), 1 μL BSA (20 mg/mL; NEB; #B9200S) and 81 μL H2O. ..

    Generated:

    Article Title: Improved Cas9-targeted nanopore sequencing facilitates ultra-deep analysis of genomic variation.
    Article Snippet: .. The complete reaction was used for Cas9 cleavage and dA-tailing using the previously generated Cas9 RNPs, Taq polymerase (NEB M0273), and 100 mM dATP solution (NEB N0440S) as per ONT’s instructions. ..



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